The localization and expression of prolactin receptor from inner mongolia alpas cashmere goat were studied by sacpic staining , in situ hybridization and western blotting . samples of skin were taken at interval three months from birth , three months old , six months old , nine months old , ten months old or twelve months old , which correspond to summer , autumn , winter and spring . paraffin sections of hair follicles were stained with sacpic staining and in situ hybridization . the protein of prolatin receptor is abstracted from samples of skin in order to study on expression of prolactin receptor . there are prolactin receptors in outer root sheath , dermal papilla and inner root sheath . the growth of primary follicle is continuous 本實驗從絨山羊出生后每隔三個月采一次皮樣,共分為4個月齡( 3 、 6 、 9 、 10或12 )段,通過制作石蠟切片,原位雜交、染色,并提取皮樣蛋白做westernblotting等實驗研究方法,研究了催乳素受體mrna催乳素受體在不同生長季節(jié)的內蒙古阿爾巴斯白絨山羊皮膚毛囊中的定位與表達,染色結果發(fā)現(xiàn)阿爾巴斯白絨山羊初級毛囊全年持續(xù)生長,次級毛囊的生長情況隨季節(jié)而變化,秋冬季生長旺盛,夏季生長緩慢與絨毛生成規(guī)律呈正相關。